Fig. 5. Role of Trx1 in SOCS1-mediated reduction in ROS and EMT signaling induced by FIR. (A) HA vs HA-SOCS1 HCT116 p53⁺/⁺cells treated with FIR were analyzed for expression levels of antioxidant proteins by immunoblot. (B) The HA vs HA-SOCS1 cells were additionally transfected with sh control or shRNA against Trx1, and analyzed for intracellular ROS levels after FIR treatment at indicated time points (**, p ≤ 0.01; ***, p ≤ 0.005). (C) The shTrx1-transfected cells were analyzed for EMT marker expression levels upon FIR treatment.